G2041

Sigma

 

Panorama® Mouse Cytokine Gene Arrays

This product number has been discontinued but a similar product may be available. Please select the recommended replacement product below (if a direct replacement is available) or use the links to "similar products" to see other possible replacement products.

Details

Related Products

References

Reviews

Description

ApplicationCytokine signaling is mediated by interaction among a wide variety of gene families. The set of arrayed genes are grouped into the following functional categories:
• Adhesion Molecules
• Angiogenic Factors
• Apoptosis-Related Factors
• Cell Surface Proteins
• Chemokines and their Receptors
• Cytokines and Cytokine Receptors
• Developmental Regulators
• EGF Family Ligands and Receptors
• Eph Family Ligands and Receptors
• SGS Family Ligands
• Integrins Family
• Interleukins and Receptors
• Intracellular Signaling Molecules
• Neurotrophic Factors
• Nitric Oxide Metabolism
• Proteases
• Telomerase Family
• TGF Superfamily
• TNF Superfamily
• Weight Regulation
• Housekeeping Genes - Negative and Positive Controls
Features and Benefits• Comprehensive collection of cytokines and other immunoregulatory factors
• Monitor changes in expression of hundreds of genes in a single experiment
• Low-cost, reusable membranes that can be analyzed by standard radiolabeling and hybridization technique
• Gene arrays are analyzed by autoradiography or phosphorimaging and easily quantified using phosphorimager software
• Signals from all the spots can be normalized to the signal of a housekeeping gene known not to change between the samples or to the averaged signal from several or all housekeeping genes
Other NotesThe Panorama™ Mouse Cytokine Gene Array is a comprehensive collection of arrayed genes encoding cytokines, chemokines, their receptors and other related immunoregulatory factors. The gene arrays provide a rapid way of determining changes in gene expression during cellular responses to developmental cues, physiological or pathological conditions, or external stimuli. Data obtained from a single experiment with the arrays is equivalent to performing hundreds of northern blots or RT-PCRs.
 The Mouse Cytokine Gene Arrays are developed in collaboration with R&D Systems, Inc.
PackagingSet of two 12x8 cm arrays containing 10 ng of each PCR amplified DNA (derived from sequenced verified cDNA clones).
PrincipleThe Panorama Mouse Cytokine gene array consists of a comprehensive collection of cytokine genes and related factors. Each Mouse Cytokine Gene Array has 514 mouse cDNAs amplified from within the protein-coding regions of the genes. Ten nanograms of PCR amplified DNA derived from sequence verified cDNA clones are represented as duplicate gene spots in the array. The genes are arrayed on charged nylon membranes that are 12 × 8 cm in size. Included in the array are 8 housekeeping genes, 5 different negative controls, and mouse genomic DNA. The housekeeping genes serve to normalize gene expression in an array, making it possible to monitor relative changes in gene expression between the control and test sample(s) on different filters. Three E. coli genes, pUC19 plasmid and TE buffer spots serve as negative controls for hybridization while the mouse genomic DNA spots serve as a positive control for hybridization. The housekeeping genes make it possible to compare relative changes in gene expression between control and test samples hybridized to two separate arrays.
Legal InformationPanorama is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

Safety

WGK Germany3

Related Products

No Related Products